Figure 5
Neuraminidase and pronase treatments differentially affect rolling and adhesion under shear stress on E- and P-selectins. MM1SHeca452 (A,B) and RPMI8226Heca452 (C,D) cells were pre-treated/mock-treated with either neuraminidase (1 mU/ml) or pronase (1 mg/ml) for 45 min at RT and then tested in a rolling and adhesion assay under shear stress on recombinant E-selectin (A,C) and P-selectin (B,D), both at 15 µg/ml. Histograms represent the mean + sem of three independent experiments performed in duplicate. Symbols on top of the error bars represent the statistical significance of the rolling (white) or adhesion (grey) fractions compared to the same fractions in the control treatment. Symbols on top of the horizontal bars represent the statistical significance of the total fraction (rolling plus adhesion fraction). The two-way ANOVA followed by Sidak’s multiple comparison post-hoc testing was used to determine statistical significance. *** p < 0.001; ** p < 0.01; * p < 0.05; ns non-significant.
