Figure 6

Expression of tight junction proteins in the rat colon and ileum. Immunohistochemical staining of the claudin-1, occludin, and ZO-1 proteins in the rat colon (a) and ileum (b). Quantitative analysis of claudin-1, occludin, and ZO-1 protein expression in the rat colon (c) and ileum (d) (N = 3 per group). The protein expression of claudin-1, occludin, and ZO-1 in the rat colon (e) and ileum (f) was calculated by western blotting. Depicted western blots are representatives from three independent experiments. Quantification of the relative intensity of the western blot images of colon (g) and ileum (h) were represented as the mean ± SD. Bars, SD; ^**P ≤ 0.01, ^***P < 0.001 versus Blank; ^#P < 0. 05, ^##P < 0. 01; ^###P < 0.001 versus Control.