Figure 1 | Scientific Reports

Figure 1

From: Structural and biochemical rationale for Beta variant protein booster vaccine broad cross-neutralization of SARS-CoV-2

Figure 1

Ancestral and Beta spike antigens: Constructs and cryo-EM particle visualization. (A) Schematic of SARS CoV-2 S-glycoproteins. Structure of native spike (A1) from the Wuhan Hu-1 isolate, ancestral spike, with amino acids and region modified in cyan (A2), Beta spike antigen with additional modifications in blue boxes (A3). The numbering of the deletions and point mutations is based on the Wuhan Hu-1 full-length protein sequence. SS, signal sequence; NTD, amino terminal domain (14–305); RBD, receptor binding domain (331–527); SD1/SD2, C-terminal domains 1 and 2 (528–685); S1/S2 protease cleavage site (685); FP, fusion peptide (816–855); FPR, fusion peptide region (856–911); HR1, heptad repeat 1 (912–984); CH, central helix (985–1034); CD, connector domain (1076–1141); HR2, heptad repeat 2; TM, transmembrane domain; CT, cytoplasmic tail. (B) Cryo-EM representative images and most populated 2D-classes for both antigens after long storage at 4ºC and after temperature treatment (37ºC for 1 day).

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