Figure 7
HDX-MS analysis. (A) Dynamic analysis of temperature treated Beta trimer at 37 °C for 24 h: Heat map analysis of Beta spike antigen comparing cold stored vs temperature treated. Sequence coverage resulting from HDX-MS was 93.4%. The magnitude of difference is demonstrated in a red-blue spectrum format. Red and blue represent increase and decrease in deuterium uptake for temperature treated sample respectively. Type II exchange peptides are highlighted in cyan whereas Type III exchange peptides are highlighted in blue. (B) Dynamic analysis mapped onto 3D structure. The HDX data for temperature treated Beta spike antigen mapped onto representative 3D structure (PDB: 7LYQ). Type II exchange regions are highlighted in cyan. Type III exchange regions are highlighted in blue. The kinetic plot of representative peptides in those regions are also demonstrated and colour coded accordingly. In the kinetic plot, cold treated sample is presented in blue whereas heat treated sample is shown in red. (C) Close look at residues 537–550: RBD down conformation is shown on the left and RBD up conformation is shown on the right. Type II exchange region (residues 537–550) is shown in cyan. For simplicity, only one protomer is shown.
