Figure 2
The initial quality control process of the read count results. (A) Relative log expression plot revealed no notable batch effects. The read counts from each area of interest are shown using box plots in the same order as the samples mounted on the experiment slides. Each slide included 12 area-of-interests from four cases with three glomeruli each (red, M1 cases; blue, M0 cases; green, control donor-kidney). (B) Kidney microstructure-specific gene expression profiles indicated a relative enrichment of glomerulus-specific genes (PODXL, NPHS2, and NPHS1) when compared to proximal convoluted tubule (SLC34A1), distal convoluted tubule (SLC12A3), and collecting duct (AQP2)-specific genes. To show the differences between other gene expression amounts, PODXL box plot was cropped and the glom-specific gene expression was evidently abundant. (C) Principal component analysis showed certain differences between the study groups (red, M1 cases; blue, M0 cases; green, control donor-kidney), which was particularly prominent for the M1 cases.
