Table 3 Input parameters for thresholding and segmentation in CellProfiler for pixel-based cell counting.
Function | Input parameter | Epidermis | Dermis | |||||
|---|---|---|---|---|---|---|---|---|
Nuclei | CD3 | CD4 | Nuclei | CD3 | CD4 | CCR5 | ||
Adaptive thresholding | Block size | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
Adaptive thresholding | Subtraction value |  − 5 |  − 45 |  − 10 |  − 30 |  − 40 |  − 35 |  − 20 |
Analyze particles | Particle size |  > 5 |  > 7 |  > 10 |  > 5 |  > 10 |  > 5 |  > 10 |
