Figure 1
From: Biological effects of Lippia alba essential oil against Anopheles gambiae and Aedes aegypti
Steps to perform the non-contact repellency test. ① : HITSS metal cylinders, ② : Linking section, ③ : drum, ④ : Rigid transparent film, ⑤ : Insect screen, ⑥ : Whatman paper (30 × 10 cm), ⑦ : Control solution, ⑧ : EO solution, ⑨ : Mosquitoes. (a) Paper impregnation, (b) positioning paper around drum, (c) connecting the cylinders and introducing the mosquitoes into the treated cylinders, (d) opening of the link section doors after 30 s of acclimatation, (e) link section doors closed after 10 min and mosquitoes counted.
