Figure 1 | Scientific Reports

Figure 1

From: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues

Figure 1The alternative text for this image may have been generated using AI.

Generation of heterozygous KCNH2 mutations in hiPSCs. Targeting strategy to independently generate KCNH2 N588D and N588K missense mutations in the 409B2 hiPSC background with mixed ssODN repair templates, and Sanger sequences of heterozygous mutant clones LQT26 and SQT22. “ssODN M" carries the representative missense mutation, either recreating KCNH2 c.1762A>G or c.1764C>A, while “ssODN B” generates c.1765G>A, a silent Cas9-blocking mutation.

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