Figure 4
Acacetin upregulated expression levels of p53 and miR-34a, while downregulated expression levels of PD-L1 of NSCLC in vitro and in vivo. (A, B) A549 and H460 cells were treated 0, 10, and 15 μM of acacetin for 48 h, mRNA levels of p53, miR-34a and PD-L1 were detected by Q-PCR (A), and protein levels of PD-L1 were detected by western blotting (B). (C, D) mRNA levels of p53, miR-34a and PD-L1 were detected by Q-PCR (C), and protein levels of PD-L1 were detected by western blotting (D) of A54-Xenograft tumor tissues. (*, p < 0.05; **, p < 0.01; ***, p < 0.001, compared with 0 μM group or 0 mg/kg group; GAPDH was used as an internal control for western blotting).
