Figure 5
Knockdown of p53 reversed the induction effects of acacetin on miR-34a expression and abolished the inhibitory activity of acacetin on cell proliferation in A549 cells. A549 cells were transfected with control siRNA (siCtrl) or p53 siRNA (siP53) for 24 h, then cells were treated 0 μM or 15 μM of acacetin for 48 h, (A & B): mRNA expression levels of p53 (A) and miR-34 (B) of each group were detected by Q-PCR; (C) cell viability of each group were detected by MTT assay. (*, p < 0.05; **, p < 0.01; ***, p < 0.001, compared with siCtrl group).
