Figure 4

Antibodies to the non-repeat region of PyCSP do not affect the ID-RAS trapping. (A) Experimental design of prime-and-trap studies. (B) Four hours after trapping with RAS IV (100 μL) or ID ULV (2.5 μL, X2 injections) + / − 7DW8-5 livers (left) and popliteal draining lymph nodes (PO dLN) (right) were excised and processed for RT-PCR to measure parasite burden with 18S pan Plasmodium primers. RT-PCR data are shown as absolute 18S rRNA copy numbers based on absolute calibrator. Error bars represent SD of mean of N = 10 mice across two independent experiments. PO dLN samples were collected from the injected side and samples were processed in two pools of N = 5 alike dLN. RT-PCR data was analyzed with Kruskal–Wallis test with Dunn’s multiple comparisons, ***p < 0.001, *p < 0.05, ns p > 0.05. (C,D) Results of protection studies after challenge with 1 × 10³ WT purified Py spz administered four weeks (C) or four months (D) after trapping with RAS + / − 7DW8-5 administered IV or ID ULV (2.5 μL, X2 injections). Protection data from N = 8–10 mice across two independent experiments and was analyzed with Fisher Exact Test, **p < 0.01, ns p > 0.05.