Figure 5
High titers of exogenously-administered spz neutralizing mAb inhibit prime-and-trap vaccination. (A) Experimental design of prime-and-trap studies. (B) Results of protection studies after challenge with 1 × 103 WT purified Py spz administered four weeks after trapping with RAS + / − 7DW8-5 administered IV or ID ULV (2.5 μL, X2 injections). RAM2 or isotype control mAb was injected IP into mice 24 h prior to trapping as indicated. Protection data from N = 10 mice across two independent experiments and analyzed with Fisher Exact Test, **p < 0.01. (C) Results of anti-RAM2 serum ELISA from a subset of the (B) mice that received RAM2 or Isotype control. Error bars represent the SD of the mean of N = 10 mice from two experiments. ELISA data was analyzed with Mann–Whitney Test, ****p < 0.0001.
