Figure 2 | Scientific Reports

Figure 2

From: Machine learning enables non-Gaussian investigation of changes to peripheral nerves related to electrical stimulation

Figure 2

Window-based structural metrics. Steps in the automated process of instance segmentation of nerve fibers' axon and myelin (A–D). (B) Fascicles manually masked in dark gray with extrafascicular space and other exclusion regions in black. (E) Manually calculating fiber density (# fibers/window area) using the quadrat method to count fibers. (F) Fiber density measured by NeMeAn (# fibers/fascicle area) using fraction of fiber area in window to count fibers. Automation also enabled calculation of the packing (tissue area/fascicle area) of axons and myelin within the windows (G & H respectively).

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