Figure 1 | Scientific Reports

Figure 1

From: Inducing positive inotropy in human iPSC-derived cardiac muscle by gene editing-based activation of the cardiac α-myosin heavy chain

Figure 1

Expression of MyHC isoforms in control hiPSC-CMs in vitro. (A) Immunofluorescence detection of the expression of endogenous β-MyHC protein (top left panel) and co-labeling with cTnT (top right panel). In lower panels, there is no detection of α-MyHC by immunofluorescence (left panel); red is cTnT (right panel). (B) Western blot demonstrating the absence of endogenous α-MyHC expression and the detection of endogenous β-MyHC in hiPSC-CMs. Adult human and rat myocytes were used as positive controls for β-MyHC and α-MyHC isoforms, respectively. Total myosin expression is shown by MF20. α-actinin is used as loading control. Weeks of beating hiPSC-CMs prior to Western blot analysis is shown at the bottom. Full blots are shown in supplemental Fig. S2.

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