Figure 6
Goblet cells are altered in the medial colon but not in the ileum or distal colon. Intestinal tissue was stained with Alcian Blue to label mucins within goblet cells and counterstained with nuclear fast red. Representative images of the distal third of the small intestine, the ileum, from (A) 1 d sham and (B) 1 d CCI mice. (C) Counts of goblet cells per villi were equivalent for sham and CCI groups but varied across timepoints. Representative images of colon tissue from both the (D, E) medial and (G, H) distal colon of 1 d sham and 1 d CCI mice, respectively. (F) CCI increased goblet cell density in the medial colon at 1 d, (G) but no effect of injury was observed in the distal colon. A main effect of time post-injury was present in both regions of the colon. Whiskers represent minimum and maximum, box represents 25th–75th percentile, and line represents median value (*p < 0.05; nsham = 3–6/timepoint; nCCI = 6–10/timepoint).
