Table 1 Characteristics of the commercial OCTA instruments and datasets.

From: Towards standardising retinal OCT angiography image analysis with open-source toolbox OCTAVA

Instrument, manufacturer

Cirrus 5000, Carl Zeiss Meditec AG24

Revo NX 130, Optopol Technology25

RTVue-XR Avanti, Optovue Inc.26*

Spectralis OCT2 module, Heidelberg Engineering27

Data collection city, country

Hong Kong, China

Bydgoszcz, Poland

Perth, Australia

Hong Kong, China

Image quantity/number of participants

32

30

31

27

Instrument definition of analysed retinal layer

SRLS

SVP

SVP, total retina**

SVC

Defined retinal layer segmentation upper boundary

ILM

ILM

ILM (SVP)

ILM (total retina)

ILM

Defined retinal layer segmentation lower boundary

ILM + 70% \(\times\) TILM-OPL

IPL (offset: -15 µm)

IPL (offset: -10 µm) (SVP)

OPL (offset: +10 µm) (total retina)

IPL (offset:  -17 µm)

Image size [mm]

3 \(\times\) 3

3 \(\times\) 3

3 \(\times\) 3

3 \(\times\) 3

Number of A-scans along x-axis

245

320

304

512

Number of B-scans along y-axis

245

320

304

512

Central wavelength [nm]

840

850

840

880

Imaging speed [A-scan rate, Hz]

68,000

130,000

70,000

85,000

Axial resolution [µm]***

5

5

5

5.7

Transverse resolution [µm]

15

18

22

11.4

Sampling density [1]****

1.23

1.92

2.23

1.95

Projection artefacts removal function

Yes

No

Yes

Yes

Angiogram construction algorithm

Optical micro-angiography (OMAG)28

Spectral domain absolute complex difference29

Split-spectrum amplitude-decorrelation (SSADA)30

Full-spectrum amplitude decorrelation31

En face projection

Maximum intensity projection

Average intensity projection

Maximum intensity projection

Sum projection + contrast function

Automated image quality indicator

Yes

Yes

Yes

Yes

Instrument name of the quality indicator

Zeiss signal strength

Quality index

Scan quality index

Q score

Image quality indicator threshold (range)

 > 6 (0–10)

 > 6 (0–10)

 ≥ 6 (0–10)

 > 15 (0–40)

Interscan time [ms]

Not specified

Not specified

Not specified

Not specified

Maximum detectable blood flow velocity [mm/s]

Not specified

Not specified

Not specified

Not specified

Instrument defined ocular axial length [mm]*****

24.46

22.10

23.95

24.39

En face image exported from the OCTA instrument: number of pixels and image type

717 \(\times\) 717 tif

640 \(\times\) 640 bmp

304 \(\times\) 304 png

1000 \(\times\) 1000 bmp

  1. SRLS superficial retinal layer slab, SVP superficial vascular plexus, SVC superficial vascular complex, ILM internal limiting membrane segmented layer, TILM-OPL thickness between ILM and the outer plexiform layer (OPL), IPL inner plexiform layer.
  2. *Two sets of data for each participant acquired in one imaging session were used to calculate intrasession repeatability for metrics generated by OCTAVA and in-built software.
  3. **OCTA image of the total retina was used only for foveal avascular zone analysis.
  4. ***Resolution is measured in tissue.
  5. ****Sampling density is defined as transverse resolution divided by scan separation, which is the transverse scan length divided by the number of samples (either number of A-scans along the x-axis or B-scans along the y-axis.
  6. *****Corrected for transverse image magnification error using the Littmann-Bennett formula as per Ref.19.
  7. Information about the instrument-defined axial length from personal correspondence (Revo NX 130 and RTVue-XR Avanti) and32 (Cirrus 5000 and Spectralis OCT2) Unit of 1 indicates dimensionless.