Figure 7 | Scientific Reports

Figure 7

From: In situ monolayer patch clamp of acutely stimulated human iPSC-derived cardiomyocytes promotes consistent electrophysiological responses to SK channel inhibition

Figure 7

Effects of 100 nM UCL1684 on iPSC-MLs. (A) Representative traces from RA-iPSC-MLs (Ai) and DMSO-iPSC-MLs (Aii) paced at 1 Hz under control conditions (black) and following application of 100 nM UCL1684 (red/blue). (B) Effect of 100 nM UCL1684 on MDP (Bi) and APD (Bii) in RA-iPSC-MLs (reds) and DMSO-iPSC-MLs (blues). The circles with black lines represent the mean of all batches. Statistics represent paired Student’s t-tests comparing APD before and after drug application. (C) Change in MDP (Ci) and APD (Cii) in iPSC-MLs following application of 100 nM UCL1684. Data are presented as mean ± SEM. Statistics represent unpaired Student’s t-tests comparing the effects on RA- and DMSO-iPSC-MLs. For all figures, different shades represent the three independent differentiation batches. Batches were pooled for comparisons between RA and DMSO. n = 12 for both groups, consisting of 4 from each differentiation batch. All recordings made at 37 °C.

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