Figure 3 | Scientific Reports

Figure 3

From: Drosophila expressing mutant human KCNT1 transgenes make an effective tool for targeted drug screening in a whole animal model of KCNT1-epilepsy

Figure 3

WT and mutant KCNT1 currents recorded in HEK293T cells. (a) KCNT1 currents recorded in HEK293T cells in response to the voltage steps ranging from − 120 mV to 80 mV in 20 mV increments followed by a voltage step to 0 mV. (b) Examples of the I-V plots of WT and mutant KCNT1 currents recorded in response to 100 ms voltage ramps between − 120 and 120 mV. For comparison, an example of the I-V plot recorded in non-transfected HEK293T cell is shown. The vertical dash line corresponds to 10 mV. (c) The average amplitudes of the WT and mutant KCNT1 currents measured at 10 mV using the I–V plots recorded in response to the voltage ramps between − 120 and 120 mV, similar to those shown in panel (b). Brown-Forsythe and Welsh’s one-way ANOVA followed by Dunnett's multiple comparisons test indicated that currents produced by the mutant constructs were significantly larger, compared to WT KCNT1 (P = 0.0003 (G288S); P = 0.0017 (R398Q) and P = 0.0026 (R928C)).

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