Figure 2 | Scientific Reports

Figure 2

From: Elemental sulfur concentration can be used as a rapid, reliable, and cost-effective predictor of sulfur amino acid content of soybean seeds

Figure 2

Panel A: SDS-PAGE analysis of 50% isopropanol-extracted proteins from mature soybean seed. Equal amounts of dry soybean seed powder were extracted with 50% isopropanol and the recovered proteins were resolved on 15% SDS-PAGE gels. Proteins were visualized by staining with Coomassie Brilliant Blue. Panel B: Immunological detection of leginsulin in soybean seeds. Isopropanol-extracted proteins were first separated on a 15% SDS-PAGE and were electrophoretically transferred to nitrocellulose membranes and probed with soybean leginsulin antibodies. Immunoreactive proteins were detected using anti rabbit immunoglobulin G (IgG)–horseradish peroxidase conjugate followed by chemiluminescent detection. The exposure time was 30 s. An empty lane between high sulfur and low sulfur lines was deleted and merged (indicated with a black dividing line). Complete uncropped digital images used for creating (A) and (B) are included in the supplemental file. Lane 1, PI603162; lane 2, PI603910B; lane 3, PI549045A; lane 4, PI437377; lane 5, PI468919; lane 6, PI082278; lane 7, PI339734; lane 8, PI424078; lane 9, PI096322; lane 10, PI229327; lane 11, PI507411; lane 12, PI603599A. The position and sizes of protein markers in kilodaltons are shown at the left of the figure.

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