Figure 2 | Scientific Reports

Figure 2

From: The Ykt6–Snap29–Syx13 SNARE complex promotes crinophagy via secretory granule fusion with Lamp1 carrier vesicles

Figure 2

Ykt6 and Vamp7 R-SNAREs differentially regulate the acidification of maturing secretory granules. (a) In control salivary gland cells, maturing SGs become positive for LysoTracker Red, and their Sgs3-GFP fluorescence is quenched due to the acidic milieu. Loss of either of the two R-SNAREs impacted the proper acidification of maturing SGs differently. The absence of ykt6 (b) did not have a statistically significant effect on the size of LTR+ structures. In contrast, the loss of vamp7 (d) strongly inhibited the acidification of maturing SGs compared to the corresponding control (c), resulting in the accumulation of smaller (presumably fusion incompetent) LTR+ lysosomes. (e,f) Quantification of the size of LTR+ structures shown in (a–d), n = 250 LTR+ structures from 5 cells of 5 different larvae, ****p < 0.0001, ns p > 0.05.

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