Figure 1

(a) The fitted standard quantitative curve, the log quantity of library concentrations (10^–3–10^–8 μM) was linearly correlated with Cq value, R² = 0.998; the error bars correspond to the standard deviation from triplicate measurements (n = 3). (b) The retention ratio of rounds 1–7. (c) The amplification curves for purified products of rounds 1–7. The curves were translated on the x-axis to improve the contrast. (d) The melting curves for purified products of rounds 1–7. The curves displayed fluorescent variation following increased temperature. (qPCR melting program: 95 °C for 10 s, 65 °C for 60 s, 97 °C for 1 s). The qPCR experiment was conducted on a Light Cycle^®96 instrument (Roche, Switzerland).