Figure 2

Plasma cortisol levels after an acute stress test in Atlantic salmon (Salmo salar ssp. bleke) originating from landlocked population in the River Otra. Fish were exposed to either gas super saturation (GSS; pH 6.5 and a total gas pressure (TGP) of 110%), simulated environmental acidification (SEA; pH 5.5 + Al; and TGP 100%), the combination of GSS and SEA (GSS + SEA; pH5.5 + Al and TGP 110%) or Hatchery river Otra water (Control; pH 6.5 and TGP 110%), for 6 days. For SEA, H2SO4 was used to acidify the water and AlCl3⋅6H2O was added to a nominal concentration of 67 μg [Al] l−1. Values are means ± standard error of mean, different letters indicate significant differences (p < 0.05) and n = 10 in each group.