Figure 2
From: C5a–C5AR1 axis as a potential trigger of the rupture of intracranial aneurysms
Up-regulation of C5AR1 in human intracranial aneurysm lesions. (A) The increase in tag count of C5AR1 in human intracranial aneurysm (IA) lesions. IA lesions (Aneurysm, n = 4) or control arterial walls (Superficial temporal artery, n = 4) were harvested subjecting to comprehensive gene expression analyses. The tag count of C5AR1 from acquired data sets is shown. Each bar indicates the actual tag count in each specimen. (B) Expression of C5AR1 in IA lesions. IA lesions (Aneurysm) or control arterial walls (Superficial temporal artery) were harvested subjected to immunohistochemical analyses. The images of immunofluorescent staining of control arterial walls or IA lesions for C5AR1 (green), nuclear staining by DAPI (blue), and merged images are shown. Scale bar: 50 μm. (C) Immunohistochemical analyses investigating cell types expressing C5AR1 in IA lesions. The images of immunofluorescent staining of control arterial walls (Superficial temporal artery) or IA lesions for C5AR1 (green), a marker for endothelial cells, CD31 (red), a marker for smooth muscle cells, α-smooth muscle actin (SMA, red), a marker for neutrophils, Myeloperoxidase (red), and merged images with nuclear staining by DAPI (blue) are shown. Scale bar: 50 μm.
