Figure 5
From: C5a–C5AR1 axis as a potential trigger of the rupture of intracranial aneurysms
The absence of the deposition of complement C3 or C5b-9 complex in intracranial aneurysm lesions. (A) Intracranial aneurysm (IA) lesions induced in rats were harvested subjected to immunohistochemical analyses. The images of immunofluorescent staining of IA lesions for C3 (green in the left panel), C5b-9 (green in the right panel), and merged images with nuclear staining by DAPI (blue) are shown. Scale bar: 50 μm. (B) The deposition of complement C3, C5 or C5b-9 complex in the kidney from the anti-Thy1.1-induced glomerulonephritis model of rats. Glomerulonephritis was induced in rats by the injection of anti-Thy1.1 antibody, and the kidney was harvested subjected to immunohistochemical analyses. The images of immunofluorescent staining of kidney with glomerulonephritis for C3 (green), C5 (green), C5b-9 (green), Thy1.1 (red), and merged images with nuclear staining by DAPI (blue) are shown. The images from immunostaining without the primary antibodies are also shown as a negative control. Scale bar: 50 μm. This experiment was done to validate the specificity of antibodies used in (A).
