Figure 1
From: Vascularised cardiac spheroids-on-a-chip for testing the toxicity of therapeutics
Overview of embedding conditions. (A) CMEF spheroids were cultured in ULA plates (suspension), in ULA plate with fibrin (fibrin) and in a microfluidic device (vascularised µFC). In the device schematic: 1 is the medium channels, 2 is the vascularised gel chamber and 3 is the central cell culture well for the vascularised spheroid. BF images show the spheroids at day 1 and day 10 of the experiments. Scale bar is 100 µm. Confocal images show vascularised CMEF spheroids after 10 days of culture in the device. Scale bar is 500 µm. (B) Confocal images of vascularised spheroids embedded at early or late time points in the devices and vasculatures formed of HUVECs or HUVECs/pericytes (medium supplemented with VEGF). Images are z-projections, while inset are single plane. Scale bar is 100 µm.
