Figure 6
From: Vascularised cardiac spheroids-on-a-chip for testing the toxicity of therapeutics
Functionality of the spheroids in suspension and in the device. (A) Beat rates (beats/ min) were assessed during 25 days of culture, in suspension and in µFCs. Statistical analysis compares beat rates of spheroids in suspension and in µFCs at corresponding time points Error bars are standard errors, n ≥ 3. For “suspension”, at least 5 biological repeats were done for each day up to 10, while 3 biological repeats were done for each day after 10. For the “µFC”, at least 19 biological repeats were done for each day up to 10, while at least 3 biological repeats for each day after 10. One technical repeat was done for each condition as one spheroid was analysed per repeat. (B) Left, FITC-dextran (10 kDa) assay demonstrating perfusability of the vascularised spheroids; Centre, confocal image of the same vascularised spheroid (scale bar is 500 µm); The red arrows indicate a structure that is proposed to correspond in the Dextran perfusion assay and the immunostaining image; Right, the vasculature generated in µFCs displays clear podocalyxin staining throughout, further confirming lumenisation of the network. Scale bar is 100 µm.
