Figure 4
Single-cell spatial mapping of spatial transcriptomic data enables identification of individual cell types in normal liver. (A) Proportion of each cell type in the snRNAseq sample and proportion of cells per spot of each cell type in the spatial sample. The distribution in both samples is similar, affirming that the proportion is taken into consideration during the single-cell spatial mapping process. (B) H&E stained tissue section after annotation by a pathologist, with red circles corresponding to central veins and blue circles to periportal regions. (C) Spatial visualization of single-cell clustering after single-cell spatial mapping overlayed on H&E stain. (D) Overlay of single-cell clustering after single-cell spatial mapping visualized on H&E section for each cell type. CV central-venous hepatocytes, HSC hepatic stellate cells, IZ interzonal hepatocytes, LSEC liver sinusoidal endothelial cells, NK natural killer cells, PP periportal hepatocytes. All figures were created in R (version 4.2.2).
