Figure 6
Cluster interaction analysis illustrates the difference in ligand–receptor analysis in BA with advanced fibrosis when comparing snRNAseq and spatially resolved transcriptomic data. (A) L–R pairs in interacting cell types show a decrease in signaling in BA liver with advanced fibrosis when compared to normal liver tissue. The three distinct colors represent whether interactions were only downregulated in snRNAseq data (blue), only in the spatially resolved transcriptomic data (yellow), or in both datasets (red). Two representative cell–cell interactions are shaded in light blue. The first indicates downregulated L–R signaling present in both datasets (red dot, spatially visualized in panel C), and the second one indicates downregulated L–R signaling present only in the single-cell spatially mapped dataset (yellow dot, spatially visualized in panel D). (B) Only 34.5% of downregulated L–R signaling pairs are present in both snRNAseq and spatial transcriptomic datasets (Spearman correlation = 0.57). 51% of downregulated L–R signaling pairs are only present in the spatial data, while 14.5% are only present in the snRNAseq data. CV central-venous hepatocytes, HSC hepatic stellate cells, IZ interzonal hepatocytes, LSEC liver sinusoidal endothelial cells, NK natural killer cells, PP periportal hepatocytes. All figures were created in R (version 4.2.2).
