Figure 1

Dynamics of response to dual PD-1/LAG-3 blockade and immune-molecular tumor characterization. (A) Shows the timeline of response dynamics together with the time of blood sampling for the immune monitoring. A rapid antitumor activity with a radiological partial response (as illustrated by the computed tomography images of a lymph node metastases in B) was achieved at week + 9 and was paralleled by the regression of most of the skin involvement. A complete clinical response of the extensive skin localization was observed at week + 18 (C). A complete radiological response (as per RECIST v.1.1) was achieved at week + 20. The immunohistochemical characterization of the tumor microenvironment showed a zonal CD3⁺ infiltrate mainly composed of CD8⁺ cells with rare CD4⁺ and rare PD-1⁺ cells (D, images at 20 × magnification). Tumor cells stained positive for the three main known LAG-3 ligands (HLA-DR, FGL-1 and galectin-3) and for the cancer testis antigen NY-ESO-1 (E, images at 20 × magnification).