Figure 4 | Scientific Reports

Figure 4

From: Elder (Sambucus nigra), identified by high-content screening, counteracts foam cell formation without promoting hepatic lipogenesis

Figure 4

Reduction in cellular cholesterol content of foam cells by aqueous extracts of elder and bitter orange. Foam cell formation was induced in RAW264.7 by addition of aggregate LDL (agg-LDL; 200 µg/mL) for 24 h; in parallel, cells were treated with plant extracts (5–10 µg/mL) or TO901317 (10 µM). Lipids were extracted by Folch extraction and subsequently free cholesterol (A), cholesteryl esters (B) and total cholesterol levels (C) were measured by gas chromatography. Data represent three independent experiments performed in duplicates.

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