Figure 5 | Scientific Reports

Figure 5

From: Elder (Sambucus nigra), identified by high-content screening, counteracts foam cell formation without promoting hepatic lipogenesis

Figure 5

Up-regulation of ABCA1 expression by elder flower and elder leaf extracts in mouse and human macrophages. Mouse RAW264.7 macrophages (A, B) or human THP-1 monocytes differentiated towards macrophages (C–F) were treated with the indicated concentrations of cold or hot extracts of either elder flowers or elder leaves in serum-reduced medium for 24 h. TO901317 (10 µM) served as positive control. Afterwards, RNA (A–D) or proteins (E–F) were extracted and analyzed for ABCA1 expression by RT-qPCR and western blotting, respectively. Data are from experiments performed in duplicates (three to four (A, B) or two (C–F) independent experiments). (E, F) Relative protein expression is shown above blots and represent mean of data from three to four biological replicates (two independent experiments). Blots were cropped for concise presentation; original blots are shown in Supplementary Fig. S3).

Back to article page