Figure 6 | Scientific Reports

Figure 6

From: Elder (Sambucus nigra), identified by high-content screening, counteracts foam cell formation without promoting hepatic lipogenesis

Figure 6

Regulation of LXR and efflux transporters by elder flower and elder leaf extracts in human macrophages. Differentiated THP-1 cells were incubated with cold or hot extracts of either elder flowers or elder leaves at the indicated concentrations in serum-reduced medium for 24 h, followed by RNA extraction. Relative mRNA expression of ABCG1 (A, E), SR-BI (B, F), LXRα (C, G) and LXRβ (D, H) was analyzed by RT-qPCR. Data from two independent experiments performed in duplicates are shown. Protein expression was analyzed by western blot (I, J). Relative protein expression is shown above blots and represent mean of data from two biological replicates, each derived from two independent experiments. Blots were cropped for concise presentation; original blots are shown in Supplementary Fig. S4.

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