Figure 5

Housekeeping gene analysis with NormFinder. This figure shows a graph with the raw Ct-values of HKGs (ACTB, TFRC, YWHAZ, HMBS, B2M, TBP, 18srRNA) and underneath HKG ranking based on stability values. These values were calculated with the algorithm NormFinder and indicate the quality/stability of the housekeeping genes for the respective sample method. (A) shows the HKG analysis for the swabs and (B) for the mouthwashes. In (A) and in (B) are the same best three genes shown for both methods, but their order differs. For swab samples the gene YWHAZ with a stability value of 0.342 is the best gene and for the mouthwash samples the gene TBP shows the lowest value with 0.339.