Table 2 Optimized protocol.

From: Characterization of non-invasive oropharyngeal samples and nucleic acid isolation for molecular diagnostics

DNA/RNA isolation

Before optimization

After optimization

Sample prep

No washing step

Washing with 1× PBS

Digestion

Proteinase K digestion for 30 min. at RT

Proteinase K digestion for 1 h at 50 °C

Purification

–

–

Elution

Elution in 35 µl nuclease free water

Elution in 20 µl nuclease free water

  1. Overview of the optimized protocol steps in direct comparison to the original protocol.
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