Table 5 Fatty acid composition (% of total lipid) of fillet of rainbow trout during the second feeding challenge.

From: Integrated alternative approaches to select feed-efficient rainbow trout families to enhance the plant protein utilization

Time points

Group

C14:0

C16:0

C18:0

ƩSAT

C16:1

C18:1n-9

C20:1n-9

ƩMUFA

  

Myristic acid

Palmitic acid

Stearic acid

Palmitoleic acid

Oleic acid

11-Eicosenoic acid or Gondoic acid

  

(Part A)

  

Initial

FD + /RF + 

1.8278 ± 0.0005

21.0978 ± 0.0041

4.7469 ± 0.0021

64.0567 ± 0.0034

6.7998 ± 0.0006

29.5829 ± 0.002

2.4435 ± 0.0007

12.1367 ± 0.0044

  

FD + /RF-

1.7641 ± 0.0012

20.9589 ± 0.0006

4.5227 ± 0.0012

63.6167 ± 0.0119

6.5368 ± 0.0047

29.8354 ± 0.0057

2.5035 ± 0.0003

12.1967 ± 0.0009

  

FD−/RF + 

1.8039 ± 0.0022

20.355 ± 0.0084

4.4847 ± 0.0013

63.3033 ± 0.0179

6.6375 ± 0.0068

30.0193 ± 0.0049

2.543 ± 0.0031

12.4467 ± 0.0049

  

FD−/RF−

1.6676 ± 0.0016

20.3468 ± 0.0052

4.5758 ± 0.0015

62.52 ± 0.0227

6.2086 ± 0.0074

29.7205 ± 0.008

2.7045 ± 0.003

12.4333 ± 0.0036

  

FD3

FD + /RF + 

1.5575 ± 0.0006

20.0387 ± 0.0056

4.3216 ± 0.001

62.1733 ± 0.0182

6.3213 ± 0.0073

29.9354 ± 0.0179

2.655 ± 0.0002

12.0967 ± 0.0067

  

FD + /RF−

1.5853 ± 0.0028

20.0121 ± 0.0073

4.5548 ± 0.0024

62.64 ± 0.0314

6.3038 ± 0.0117

30.1837 ± 0.0133

2.8049 ± 0.0029

12.1667 ± 0.0014

  

FD−/RF + 

1.6141 ± 0.0009

19.7536 ± 0.0023

4.3777 ± 0.0013

62.5067 ± 0.0087

6.3497 ± 0.0043

30.4116 ± 0.0024

2.6569 ± 0.0016

12.56 ± 0.0026

  

FD−/RF−

1.6403 ± 0.0013

20.0445 ± 0.009

4.3634 ± 0.0025

63.1633 ± 0.0223

6.6461 ± 0.0071

30.4707 ± 0.0027

2.5501 ± 0.0017

12.3167 ± 0.0071

  

RF3

FD + /RF + 

1.6824 ± 0.0006

20.7828 ± 0.0024

4.4749 ± 0.0008

64.02 ± 0.0003

6.9248 ± 0.0014

30.1575 ± 0.0038

2.3442 ± 0.0014

12.1767 ± 0.0023

  

FD + /RF−

1.6119 ± 0.0015

20.3233 ± 0.0048

4.4961 ± 0.0017

62.6067 ± 0.0076

6.2883 ± 0.0028

29.8898 ± 0.0082

2.4546 ± 0.0026

12.2433 ± 0.0039

  

FD−/RF + 

1.6366 ± 0.0001

20.4421 ± 0.004

4.1889 ± 0.0011

61.9233 ± 0.0067

6.1797 ± 0.002

29.4775 ± 0.0054

2.5977 ± 0.0012

12.5167 ± 0.005

  

FD−/RF−

1.6453 ± 0.0009

20.5796 ± 0.0034

4.4093 ± 0.0009

62.6667 ± 0.0045

6.4838 ± 0.0026

29.547 ± 0.0058

2.3315 ± 0.0012

12.2033 ± 0.0038

  

FD4

FD + /RF + 

1.6567 ± 0.0017

20.1115 ± 0.0021

4.4487 ± 0.0013

62.8733 ± 0.0112

6.6561 ± 0.0048

30.0036 ± 0.0051

2.3654 ± 0.0024

13.1533 ± 0.0146

  

FD + /RF−

1.551 ± 0.0007

20.3422 ± 0.0025

4.4039 ± 0.0008

63.1667 ± 0.0027

6.6835 ± 0.0035

30.1842 ± 0.0023

2.2371 ± 0.0002

11.9667 ± 0.0002

  

FD−/RF + 

1.6373 ± 0.0015

20.7404 ± 0.006

4.4657 ± 0.0026

61.77 ± 0.0317

6.419 ± 0.0082

28.5057 ± 0.0308

2.4202 ± 0.0023

12.9133 ± 0.0073

  

FD−/RF−

1.6769 ± 0.0006

19.7024 ± 0.0025

4.3694 ± 0.0018

63.0433 ± 0.0086

6.8509 ± 0.0026

30.4411 ± 0.0096

2.5013 ± 0.0012

13.29 ± 0.0084

  

RF4

FD + /RF + 

1.8273 ± 0.0011

20.3163 ± 0.0042

4.5457 ± 0.0007

64.9933 ± 0.0037

7.5477 ± 0.0049

30.7573 ± 0.0044

2.4404 ± 0.0012

13.2833 ± 0.0145

  

FD + /RF−

1.6957 ± 0.0007

21.0809 ± 0.002

4.4498 ± 0.0006

65.4167 ± 0.0115

7.1762 ± 0.0028

31.0182 ± 0.0068

2.3773 ± 0.0011

12.12 ± 0.0013

  

FD−/RF + 

1.5744 ± 0.0018

20.3452 ± 0.012

4.3435 ± 0.0028

63.9167 ± 0.0383

6.8197 ± 0.0055

30.8316 ± 0.0167

2.5071 ± 0.0023

12.1933 ± 0.0073

  

FD−/RF−

1.7205 ± 0.0002

20.6855 ± 0.0037

4.3482 ± 0.0011

64.2667 ± 0.0071

6.9989 ± 0.0024

30.5132 ± 0.0053

2.4374 ± 0.0008

12.7667 ± 0.0062

  

P-value (Time point)

 

0.0265231*

0.0098728**

0.0377131*

0.03304733517*

0.0151821*

0.095079459

0.0031691***

0.174451829

  

P-value (group)

 

0.506083942

0.489478369

0.0936461

0.448398197

0.312895794

0.79230104

0.523703357

0.19903086

  

P-value (Time point × group)

 

0.723457976

0.326836156

0.455290928

0.974680216

0.879843959

0.840963884

0.441030565

0.561316147

  

Time points

Group

C18:2n-6

C18:3n-3

C20:2n-6

C20:3n-6

C20:4n-6

C20:3n-3

C20:5n-3

C22:5n-3

C22:6n-3

ƩPUFA

linoleic acid

α-Linolenic acid

Eicosadienoic acid

Dihomo-γ-linolenic acid

Arachidonic acid

Eicosatrienoic acid

Eicosapentaenoic acid

Docosapentaenoic acid

Docosahexaenoic acid

(Part B)

Initial

FD + /RF + 

8.5749 ± 0.0029

1.0236 ± 0.0008

0.3489 ± 0.0005

0.2074 ± 0.0001

0.7858 ± 0.0003

0.1774 ± 0.0002

7.5572 ± 0.0016

1.9054 ± 0.0012

12.65 ± 0.0057

23.63 ± 0.0052

FD + /RF−

8.626 ± 0.0012

1.0192 ± 0.0004

0.354 ± 0.0001

0.2136 ± 0.0002

0.8274 ± 0.0006

0.1927 ± 0.0002

7.8135 ± 0.0038

2.0549 ± 0.0019

12.5568 ± 0.0065

24.0133 ± 0.0125

FD−/RF + 

8.802 ± 0.0018

1.0099 ± 0.0004

0.3646 ± 0.0002

0.2149 ± 0.0002

0.7838 ± 0.0016

0.1632 ± 0.0007

7.7077 ± 0.0055

2.0128 ± 0.0003

12.7543 ± 0.0045

24.0033 ± 0.0124

FD−/RF−

8.6328 ± 0.0009

1.0488 ± 0.0005

0.3852 ± 0.0002

0.2064 ± 0.0001

0.8241 ± 0.0014

0.202 ± 0.0008

7.9815 ± 0.0072

2.0132 ± 0.0014

13.2769 ± 0.011

24.89 ± 0.0211

FD3

FD + /RF + 

8.4907 ± 0.0063

0.9495 ± 0.0004

0.3764 ± 0.0004

0.2256 ± 0.0001

0.8326 ± 0.0007

0.185 ± 0.0002

7.5267 ± 0.0043

2.0581 ± 0.0003

14.493 ± 0.021

25.6967 ± 0.0248

FD + /RF−

8.4015 ± 0.0029

0.9599 ± 0.0003

0.3667 ± 0.0005

0.198 ± 0.0004

0.8808 ± 0.0018

0.2109 ± 0.0006

7.6432 ± 0.0044

2.0611 ± 0.0009

13.7732 ± 0.0225

25.1333 ± 0.031

FD−/RF + 

8.907 ± 0.0011

0.9967 ± 0.0004

0.3708 ± 0.0003

0.2115 ± 0.0002

0.8489 ± 0.0009

0.231 ± 0.0004

7.2477 ± 0.0027

2.08 ± 0.0008

13.8591 ± 0.0083

24.85 ± 0.0061

FD−/RF−

8.7905 ± 0.0049

0.975 ± 0.0006

0.3711 ± 0.0002

0.2189 ± 0.0001

0.8159 ± 0.0016

0.2086 ± 0.0009

7.4789 ± 0.0041

2.0194 ± 0.0021

13.3158 ± 0.0079

24.43 ± 0.0153

RF3

FD + /RF + 

8.8042 ± 0.0018

1.028 ± 0.0014

0.3375 ± 0.0001

0.1973 ± 0.0

0.7028 ± 0.0001

0.1694 ± 0.0001

7.3327 ± 0.0024

2.1135 ± 0.001

12.9066 ± 0.0007

23.7567 ± 0.0021

FD + /RF−

8.7977 ± 0.0029

0.9887 ± 0.0004

0.3606 ± 0.0003

0.186 ± 0.0002

0.7392 ± 0.0004

0.149 ± 0.0002

7.3646 ± 0.0011

2.2102 ± 0.0008

14.1177 ± 0.0097

25.1267 ± 0.0106

FD−/RF + 

8.9079 ± 0.0036

1.0107 ± 0.0004

0.3715 ± 0.0002

0.2193 ± 0.0003

0.8296 ± 0.0003

0.1679 ± 0.0003

7.5521 ± 0.002

2.1518 ± 0.0016

14.2182 ± 0.0079

25.5133 ± 0.0099

FD−/RF−

8.8973 ± 0.0031

0.9748 ± 0.0005

0.3538 ± 0.0003

0.1856 ± 0.0001

0.7491 ± 0.0002

0.1734 ± 0.0

7.3259 ± 0.0036

2.1809 ± 0.0015

14.1412 ± 0.0119

25.11 ± 0.0081

FD4

FD + /RF + 

9.7278 ± 0.0111

1.0607 ± 0.002

0.3565 ± 0.0005

0.2058 ± 0.0002

0.7272 ± 0.0011

0.1517 ± 0.0005

6.7742 ± 0.0074

2.3004 ± 0.0025

13.4223 ± 0.0154

23.9367 ± 0.0256

FD + /RF−

8.806 ± 0.0004

0.9228 ± 0.0001

0.3312 ± 0.0002

0.1908 ± 0.0002

0.7189 ± 0.0003

0.1437 ± 0.0001

7.3174 ± 0.0017

2.2923 ± 0.0007

13.7988 ± 0.004

24.7967 ± 0.0029

FD−/RF + 

9.4863 ± 0.0051

1.0072 ± 0.0006

0.3703 ± 0.0003

0.2037 ± 0.0003

0.7726 ± 0.0012

0.1582 ± 0.0002

7.2705 ± 0.0079

2.363 ± 0.0012

14.0919 ± 0.0168

25.23 ± 0.0276

FD−/RF−

9.6922 ± 0.0074

1.0952 ± 0.0019

0.3732 ± 0.0005

0.1912 ± 0.0003

0.7021 ± 0.0003

0.1377 ± 0.0001

6.9443 ± 0.0049

2.2891 ± 0.0009

12.9846 ± 0.0058

23.6233 ± 0.0031

RF4

FD + /RF + 

9.6812 ± 0.0103

1.1603 ± 0.0031

0.3805 ± 0.0002

0.214 ± 0.0003

0.6421 ± 0.0001

0.1638 ± 0.0002

6.4907 ± 0.0068

2.1301 ± 0.0027

11.5534 ± 0.0063

21.5733 ± 0.0128

FD + /RF−

8.7725 ± 0.0019

0.9675 ± 0.0002

0.3257 ± 0.0001

0.1727 ± 0.0001

0.682 ± 0.0003

0.1659 ± 0.0002

7.0025 ± 0.0032

2.0413 ± 0.0012

11.9789 ± 0.0069

22.37 ± 0.0108

FD−/RF + 

8.6817 ± 0.0062

1.0052 ± 0.0013

0.3479 ± 0.0004

0.1971 ± 0.0003

0.6247 ± 0.0003

0.0812 ± 0.0008

7.0216 ± 0.0067

2.1747 ± 0.0023

13.3913 ± 0.0305

23.84 ± 0.0395

FD−/RF−

9.241 ± 0.0043

1.0874 ± 0.0012

0.3545 ± 0.0002

0.2041 ± 0.0002

0.6931 ± 0.0005

0.1566 ± 0.0003

6.7701 ± 0.002

2.048 ± 0.0005

12.7037 ± 0.0046

22.93 ± 0.006

p-value (Time point)

 

0.0015647***

0.449740864

0.607877716

0.135670645

0.0001178***

0.0029413**

0.0001542***

0.000064***

0.02178734*

0.01798366092*

p-value (group)

 

0.144909572

0.28838482

0.31671723

0.079557304

0.713313444

0.789716276

0.372217184

0.737278478

0.561940556

0.521070269

p-value (Time point × group)

 

0.504056685

0.885364453

0.700632472

0.683441163

0.921131768

0.545994749

0.945130189

0.983744936

0.841649354

0.935568509

  1. *, **, ***The p value less than 0.05 are significantly different among the four groups.
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