Figure 1

(A) Photography of a HA column showing the brownish product retained at the top of the column (arrow) during the chromatographic separation of F. hepatica ESAs. (B) Elution profile of F. hepatica ESAs obtained by size exclusion chromatography (Superdex 75 HR 10/30, ÄCTA LC) monitored at 280 nm. The fraction corresponding to Peak IV was used for comparative studies with fractions obtained by HAC.