Figure 6 | Scientific Reports

Figure 6

From: Increased specificity of Fasciola hepatica excretory-secretory antigens combining negative selection on hydroxyapatite and salt precipitation

Figure 6

Plot showing serum IgG responses of infected (closed circles) and non-infected (open circles) cattle to four F. hepatica antigen preparations (ESAs, HAC-NR, Fi-SOLE and Peak IV). Each point represents an individual serum. The ELISA IgG values were expressed as Log10 OD response measured at 450 nm. Horizontal red bars represent optimal cut-off values obtained by ROC analysis for each antigen (see Table 3). Horizontal blue bars represent cut-off values obtained by ROC analysis selecting 100% specificity. The lowest and highest OD values obtained for each tested antigen were as follows: (i) sera from Fasciola-infected cattle: ESAs (0.1/2.40), HAC-NR (0.26/2.34), Fi-SOLE (0.22/2.39), Peak IV (0.17/1.95); (ii) sera from Fasciola-free cattle: ESAs (0.02/1.23), HAC-NR (0.08/0.81), Fi-SOLE (0.03/0.32), Peak IV (0.03/0.20).

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