Table 5 Diversity and relative abundance of unique proteins identified by MS/MS spectrometry in F. hepatica antigen in fraction HAC-NR which were not identified in fractions Fi-SOLE or Peak IV. Individual proteins covering 0.5% or more of total HAC-NR spectra (n = 4084) were noted in bold. The spectra number of unique proteins in fraction HAC-NR was n = 156.

From: Increased specificity of Fasciola hepatica excretory-secretory antigens combining negative selection on hydroxyapatite and salt precipitation

Unique proteins

Spectra number

Percentage *

Actins [Fasciola hepatica]

27

0.66

Beta-tubulin [Fasciola hepatica]

47

1.15

Collagen type IV alpha 2 chain [Fasciola hepatica]

1

0.02

EGF region [Fasciola hepatica]

1

0.02

Fibrillin-2 [Fasciola hepatica]

1

0.02

Fructose-biphosphate aldolase B [Fasciola hepatica]

1

0.02

Heat shock protein 70 [Fasciola hepatica]

44

1.08

Histones [Fasciola hepatica]

24

0.58

Nuclear transport factor [Fasciola hepatica]

1

0.02

Tegumental CaBP4 [Fasciola hepatica]

6

0.15

Toll-interacting protein B [Fasciola hepatica]

1

0.02

Twitchin [Fasciola hepatica]

2

0.04