Figure 1
From: Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes
Phenotyping, genotyping and genetic characterization of the NSE-Noggin model. (A) NSE-Noggin HO mice have dramatic hair loss compared to NSE-Noggin WT mice and can be distinguished based on phenotype. (B) Genotyping PCR and gel electrophoresis show the presence of the transgene at 110 bp and the internal positive control for presence of DNA at 200 bp. Neg = negative control. (C) Genotyping qPCR results in different melt peaks for transgenic and WT mice. (D) The NSE-Noggin-IRES-EGFP construct is present in the NSE-Noggin HO mice based on DNA sequencing of a 1938 bp sequence indicated by arrows. The construct consists of 4 kb of rat NSE gene DNA with 2.8 kb 5′ flanking DNA, exon 1 (50 bp), intron 1 (1.2 kb), and 6 bp of exon 2, followed by the CDS of Noggin, IRES, EGFP and SV40 polyadenylation signal. (E) Noggin is significantly higher expressed in NSE-Noggin HO mice compared to NSE-Noggin WT mice confirmed by quantitative PCR analysis with two different primer sets. (F) Immunoreactivity for the EGFP reporter is present in the myenteric plexus of NSE-Noggin HO mice and absent from NSE-Noggin WT mice, confirming translation of the Noggin-EGFP construct. Scale bar equals 20 µm.
