Table 2 List of antibodies used for immunofluorescence.
From: Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes
Antibody | Host | Dilution | Supplier | Cat. no. |
|---|---|---|---|---|
Primary antibodies | ||||
GFP | Rabbit | 1:200 | Abcam | ab290 |
Hu | Human | 1:20,000 | Gift from Vanda Lennon | |
Calbindin | Rabbit | 1:20,000 | Swant | CB-38a |
Calretinin | Goat | 1:500 | Swant | CG1 |
Serotonin | Goat | 1:1000 | Abcam | ab66047 |
Secondary antibodies | ||||
Anti-human Alexa 594 | Goat | 1:1000 | Thermo Fisher | A-11014 |
Anti-human Dylight 650 | Donkey | 1:1000 | Thermo Fisher | SA5-10129 |
Anti-rabbit Alexa 488 | Donkey | 1:1000 | Thermo Fisher | A-21206 |
Anti-goat Alexa 594 | Donkey | 1:1000 | Thermo Fisher | A-11058 |
