Figure 1
From: Targeting autophagy as a therapeutic strategy in pediatric acute lymphoblastic leukemia
Activation of autophagy and lysosomal pathways in ALL cells with ETV6-RUNX1 translocation. (a) Clustering according to the cytogenetic background of 41 pre-B ALL cell lines by Principal component analysis (PCA) of highly variable genes from RNAseq data. (b) Gene ontology—Biological process analysis of up-regulated genes in ETV6-RUNX1 cell lines as compared to all the other ALL cell lines. (c) Expression levels of Vps34 (PIK3C3) and ATG14 proteins in ETV6-RUNX1-bearing cell lines compared to other pre-B ALL or T-ALL cell lines as assessed by proteomics. (d) Expression levels of pik3c3 and atg14 mRNA in St. Jude cohort and EGA (European Genome-Phenome Archive) combined large dataset consisting of 419 pediatric ALL patients (ETV6-RUNX1 t(12;21), n = 18, other pre-B-ALL, n = 401). (e) Representative Western blotting (n = 3) to assess basal autophagic flux in ETV6-RUNX1 (REH and COG-LL-355h) compared to other cell lines (RCH-ACV, MHH-CALL2 and 697) as monitored by LC3B-II levels in the presence of bafilomycin A1, BafA1. GAPDH is used as loading control. The membrane was cut after transfer according to the corresponding protein sizes. Ratio of LC3B-II to GAPDH is quantified using Image J and presented under the figure. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001 using either a two tailed t-test with Welch’s correction for two groups (d) and a 2-way ANOVA with Dunnett’s (one-to-many) multiple comparison test (c).
