Figure 4
From: Targeting autophagy as a therapeutic strategy in pediatric acute lymphoblastic leukemia
Combination of dexamethasone and autophagy inhibitors show synergistic effects in T-ALL cells. (a, b) P12-Ichikawa (a) and CCRF-CEM (b) cells were seeded in automatic manner into 384 well plates containing indicated dilutions of drugs and their combinations (besides Dexa + PIK-III combination, which was done manually in 96-well plates as in Fig. 3a, c), incubated for 72 h and cell viability was measured using CellTiter-Glo. Data was analyzed using SynergyFinder. A representative two-drug interaction surface plot for each cell line is shown (n = 2). (c) IncuCyte live-cell imaging of P12-Ichikawa and CCRF-CEM cells treated with the indicated drugs and drug combinations for 72 h (n = 3, each performed in triplicate). Green fluorescence corresponding to caspase 3/7 activity at 72 h time point was normalized to cell confluence and presented ± SEM. Treatment with 0.1 µM Staurosporine was used as a positive control for apoptosis induction. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001 using 1-way ANOVA with Šidák multiple comparison test.
