Figure 5
From: Targeting autophagy as a therapeutic strategy in pediatric acute lymphoblastic leukemia
Combination of dexamethasone and autophagy inhibitors show synergistic effects in ALL primary cells ex-vivo. (a, b) Primary ALL cells were seeded either in 384 (in duplicate) or in 96-well (in triplicate) plates (n = 11, 6 Pre-B- and 5 T-ALL) and treated with either Dexa alone or in combination with Lys-05 or PIK-III for 48 h and viability was measured using CellTiter-Glo. A representative of a pre-B- (a) or a T-ALL (b) sample; data shown as inhibition of viability curves (upper) or as two-drug interaction surface with respective ZIP synergy scores (lower); (c) Summary of data of ZIP scores (X-axis) of the indicated drug combinations (Y-axis) for the 11 primary samples (Table 3 for ZIP scores and Supplementary Table 3 for patient information). (d) Schematic illustration of Dexa-induced leukemia cell death and potentiation through inhibition of autophagy or lysosomal degradation.
