Figure 3
From: Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell
Optimization of photothermal PCR conditions. 1.5% agarose gel electrophoresis of amplicons from photothermal PCR performed with different denaturation and annealing/extension temperatures, times, and cycle numbers. Amplicons from photothermal PCR with (a) different denaturation temperatures (b) different annealing/extension temperatures (c) different denaturation times (d) different annealing/extension times (e) different cycle number. The gel images in (a)–(e) are cropped. The original gel images are presented in Supplementary Fig. S4–S8. Lane M: 100 bp DNA Ladder, Lane C: conventional PCR.
