Table 1 Composition of PCR mixture for photothermal PCR.
From: Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell
Components | Volume (μL) |
|---|---|
10X Z-taq Buffer (Mg2+ plus) (30 mM) | 1 |
Takara Z-Taq (2.5 U/μL) | 0.1 |
dNTP mixture (2.5 mM each) | 0.8 |
Lambda forward primer (10 μM) | 0.9 |
Lambda reverse primer (10 μM) | 0.9 |
Bovine serum albumin (20 μg/μL) | 0.5 |
Lambda DNA (0.5 ng/μL) | 2 |
Gold nanoshells (1.53 μg/μL) | 1 |
Ultra pure water | 2.8 |
