Figure 2

The detection of M-MDSCs in peripheral blood by flow cytometry. M-MDSCs was defined as CD14+CD33+CD45+HLA-DR− Monocyte. The analysis method as follows: (A) All karyocytes (Gate 1) were selected by SSC and CD45 expression from all events. (B) The CD33+CD45+ cells (Gate 2) were selected from Gate 1. (C) The CD14+ Monocyte (Gate 3) were selected from Gate 2. (D) CD14+ HLA-DR− Monocyte were selected from Gate 3 and the percentage of CD14+CD33+CD45+HLA-DR− Monocyte represents the M-MDSCs level. All data were acquired and analyzed by Software CytExpert (2.0).