Figure 1
From: High-dose exposure to butylparaben impairs thyroid ultrastructure and function in rats

Experimental procedure. This study included animal and cell experiments. In vivo, rats were injected subcutaneously with BuP at 50 mg/kg bw/d for 4 weeks. After euthanasia, thyroid histology, serum levels of thyroid hormones, and thyroid autoantibodies were determined. In vitro, the human thyroid follicular cell line Nthy cells were exposed to 0 to 80 mg/L BuP and cell viability, proliferation, apoptosis, and sodium iodide symporter (NIS) levels were determined.