Table 1 Buchnera genes targeting PNAs.

From: In vivo interference of pea aphid endosymbiont Buchnera groEL gene by synthetic peptide nucleic acids

PNA designation

PNA sequence

Tm (at 4 μM; °C)

Targeting gene

Target site

Off-target gene

PNA_GroEL

(RXR)4XB*-O-gccatttgac-NH2

55.6

groEL

− 5 to + 5

pyrG

PNA_mm

(RXR)4XB-O-gcgatttgtc-NH2**

55.8

 

–

–

PNA_GroEL_TR

(RXR)4XB-O-gccatttgac-Texas Red

 

groEL

− 5 to + 5

pyrG

  1. *In the cell-penetrating peptide conjugate, (RXR)4XB = RXRRXRRXRRXRXB, X stands for 6-aminohexanoic acid, B stands for beta-alanine while R stands for arginine.
  2. **The bold and underlined bases were changed to become mismatches to the original complementary bases of the groEL gene.
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