Figure 1 | Scientific Reports

Figure 1

From: Kinetic characterization of human mRNA guanine-N7 methyltransferase

Figure 1

Assay optimization for RNMT. (A) RNMT activity was tested at a pH range of 7.0 to 9.0 using 10 mM Tris–HCl. The effects of additives such as (B) DTT, (C) BSA, (D) Triton X-100, (E) MgCl2, (F) KCl, (G) NaCl, (H) EDTA, and (I) DMSO were evaluated using optimal pH buffer (10 mM Tris, pH 7.5). Plotted values are the mean ± standard deviation of three independent experiments. Data were analyzed using GraphPad Prism 9.

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