Figure 1 | Scientific Reports

Figure 1

From: Trypanosoma cruzi interaction with host tissues modulate the composition of large extracellular vesicles

Figure 1

Workflow of the experimental design to obtain 2 and 24 h post infection (h.p.i.) LEVs. Myoblasts (C2C12) and intestinal epithelium (Caco-2) cells were seeded and infected with T. cruzi TCTs belonging to two distinct strains (CL Brener, DTU Tc VI and Dm28c DTU Tc I). LEVs were collected in the first 2 h and after 24 h of infection. LEVs derived from isolated C2C12, Caco-2 and T. cruzi TCTs were used as controls. LEVs were lysed, their proteins were separated and digested in-gel with trypsin and the peptides were analyzed by LC–MS/MS for protein identification and quantification.

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